com 9. Unplug the power cable from the back of the instrument. If the computer is connected to the Internet: Select Search Automatically for updated driver software. 2. The SpectraMax® ABS Plus Microplate Reader can run . flexibility of monochromator-based systems. Information on how to. Five preferred detection modes. We would like to show you a description here but the site won’t allow us. GloMax® Discover is a ready-to-use multimode plate reader developed with Promega reagent chemistries to provide a simple means of detecting luminescence, fluorescence and absorbance. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. For users operating in a FDA 21 CFR Part 11 compliant environment, SoftMax Pro GxP Software is available, allowing user permissions, audit trails, e-signature and reporting tools. SpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. When a feature or capability applies to only certain readers, this exception is noted. Plate readers can vary in attributes such as microwell plate format, throughput, optical system, wavelength range, and more. In this product video, see how the SpectraMax M5 quickly reads samples. Wide range of applications. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. SpectraMax M5 reader SpectraMax M5e. e. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. No additional manuals are available. The SpectraMax iD5 reader rapidly performs absorbance measurements that are used to plot standard curves and calculate sample concentrations using a preconfigured protocol in SoftMax Pro Software. Figure 1. both cuvette-based and microplate reader applications SpectraMax M5 Plate Reader (Molecular Devices) If you have trouble accessing this page and need to request an alternate format, contact [email protected] SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. The chamber. The plate was vigorously mixed and incubated for 4 min at room temperature in the dark. Page 1 of 8 . For more information please visit us at: M 系列多功能微孔板读板机包括 M2/M2e、M3、M4、M5/M5e 等几款多功能酶标仪。. Abs). The SpectraMax ® i3x. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Review date: 07 Nov 2017 | SpectraMax® M5 Multi-Mode Microplate Reader. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or We use Spectramax M5 microplate reader in our lab. Find Molecular Devices SpectraMax M3/M4/M5/M5e Only Molecular Devices microplate plate readers offer the In need of a service manual for the SpectraMax M5 Find Molecular Devices SpectraMax Plus 384 – Forums, 190 – 1000 nm, tunable Molecular Devices SpectraMax Plus 384 Plate Reader. The chamber. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. EarlyTox Caspase-3/7 R110 Assay. Molecular devices SpectraMax M5 Pdf User Manuals. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. In other words, I carry out equilibrium folding/unfolding titrations of proteins and monitor the process using. The chamber. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The following document is. The entry should be SpectraMax GEMINI EM. Table of Contents. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. I have been using the SpectraMax M5 since 2011. #3635 or Greiner cat. The plate is set up for reading and images are acquired according to specified parameters. The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. ScanLater Western Blot Detection Cartridge. There is currently no charge for using this plate reader. Then the plate was returned to the. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. Microplate Reader, Multimode. MichiganSpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . Using the. Examples: “Press carefully to engage,” “ Do not press down. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. PAINS analysisA single-mode plate reader is a good starting point. If no sound is heard during initialization, contact technical support. 0 out of 5 $ 15,999. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. • Tel. 8. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 2. These robust readers have been placed in labs from. When the user applies a set temperature of 37°C, the top is heated to 37. Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. 000 to 3. book Page 1 Wednesday, October 12, 2011 4:56 PMThe SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. Add to Cart. An integration time of 1 second was used for each reader. 1. spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiter Created Date:1) Select the correct plate and the wells that you want to measure. Description. 303 unable to cal dark current. Plate section without PathCheck applied. Set up a plate section for a second emission scan as in Step C above, except for the added Emission Cutoff Filter. Choose from a three- (M3), four-. Considerations for readers include the measurement wavelength range, microwell plate capacity, throughput, detection methods, and a filter vs monochromator-based optical system. 40266The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. were mixed for 2 minutes using a plate shaker. Brand New. Instructions. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Third this recommendation. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. PURPOSE. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 12. BMG Rubystar High. 4. We carry both single-mode and multi-mode readers from manufacturers such as BioTek Instruments, Perkin Elmer, and SRU Biosystems. As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. The plastic from a microplate will affect the light polarization, precluding bottom reads and reading a covered plate. The performance of these two modes is not comparable to dedicated luminescence or timeresolved fluorescence instruments, or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. com 10. The Spectr. The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Microplate Reader Shelf. Five preferred detection modes. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. edu) to be added as a trained user in iLab. SpectraMax i3x Multi-Mode Microplate Re. SeePlateAdapteronpage20. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. Comes from Lab closure Approx. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. 40266 Email: [email protected] OD ± 3. no. Abs). Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Application Area: Multi mode plate reader. Setup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Access this by going to Help >. After the plate is read, data will appear in the plate section: 10. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Cells in each image are identified by SoftMax Pro Software and cell-by-cell it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode…. SeePlateAdapteronpage20. edu, 814-863-3503. Cells in each image are identified by SoftMax Pro Software and cell-by-cellit is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. 000000 out of 5. 1. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. 3752 in as per SBS dimensions. Among the various ready-to-read protocols, users will find pre-written SpectraDrop Plate protocols enabling Micro Volume applications measurement. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. ”The SpectraMax 190 reader can be integrated with Molecular . Bioz Stars score: 86/100, based on 1 PubMed citations. The SpectraMax M3 Microplate Reader is supplied with SoftMax Pro Data Acquisition and Analysis Software, Molecular Devices' industry leading all-in-one software. . ELISA Plate Readers / Microplate Readers. Pathcheck Pathlength Measurement Technology. Microplate Readers Biotek Epoch Microplate Reader. Your all-access pass to your next breakthrough. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode cuvetteportanda6-wellto384-wellplatereadcapability. These robust readers have been placed in labs from Antarctica to. spr SpectraMax Plus 384. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. 6 for further experiment, so I'm confused which i would choose for OD value determination. Performing an enzyme dilution series verifies the enzyme and assay performance and allows calculation of the EC 80 concentration of enzyme that will be used for inhibitor screening assays. The assay window spanned four orders of magnitude. Synergy H1 Hybrid Multi-Mode Reader Synergy™ H1 is a configurable multi-mode microplate reader. . 04 to 3. 3: The cuvette chamber. flexibility of monochromator-based systems. Plates were measured at 450 nm with 650 nm as a reference (Molecular Devices, SpectraMax M). com 4. Instruments with a 96- or 384-well format will accommodate the most commonly used plates. SpectraMax M2, M2e;. Description 1. Plate Shaking Linear, Orbital Slow, and Orbital Fast. Microplate Readers include the M3, M4,. 2. Gemini EM. It was then read from the top and bottom on the SpectraMax iD3 reader using a preconfigured protocol in SoftMax Pro Software with the settings indicated in Table 1. Label: provides information. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the plate drawer. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 2. Supersedes 1. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 0 OD for earlier models. Fluorescence Microplate Reader Comparison Chart. B. Wide range of applications The VersaMax reader covers a wide range of applications:Molecular Devices. Spectramax M5. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. Five. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. White plates reflect the light, enabling even low levels of Luminescence to be detected. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS control panel are shown in boxed Helvetica type. With optimized reagents, option to operate the readers. The SoftMax® Touch Software is installed in. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Instrument setup and sample analysis. Test up to 3. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. In the lower half of the window, left side, there is a box labeled reader. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. FlexStation. 18 x 14 x 8 ins. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. 304 signal level saturation. IMAP assays are based on binding of. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. 96/384 - For the SpectraMax 340PC384, press to select to read a 96-well plate or a 384- well plate. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. ". 3 or greater. Ensure data accuracy of your reader. Molecular Devices microplate readers. 40266 Email: drugdiscoverytech@lifetech. When the correct COM port is selected, the icon of the SpectraMax L reader in the upper left hand corner will be displayed in color. The advantage of using labeled probes is detection specificity. The SpectraMax M5 Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. Example: [Drawer]. Lower end of DNA standard curve. Call 516-939-0888 to check. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. SpectraMax Injector Cartridge with SmartInject™ Technology. SpectraMax i3 reader settings for IMAP PDE assays. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. For more information, please visit us at Plate-Based Assays. i3. Contact Local Team. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. 25 fmol/well, 384-well plate)−top Fluorescein 4 pM (0. 0% ± 0. 1. Multimode Readers Comparison Chart. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. 7°C and the bottom to 36. Narrow your selection of used microplate readers using the filters above or select the one of the categories on the left to view multi-mode or. com 4. Description. BMG Nephelostar Laser-Based Microplate Nephelometer Microplate Reader. 0 out of 5 $ 17,999 $ 16,995. 0397 in x 3. 3) At the bottom of the strip, click on "Show. We empower scientists to advance discovery Automated, end-to-end solutions span research disciplines to help scientists discover, scale, and industrialize biology. Designed to provide the simplicity, flexibility and power required for advanced data analysis. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Experimental details and customization of the group table. With optimized reagents and the industry-leading. Delivers single mode reader performance in one multimode reader package. Step 4. $6,649. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. 0 . Optical absorbance was measured at 734 nm on a SpectraMax M5. Reader Documentation Version No. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Description 1. UV/Vis Photometric Performance: Wavelength Range. Be sure to insert . The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 1. 0112-0115 F . SpectraMax® M5 Microplate Reader Molecular Devices LLC. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. Last lab had ours for years and never had a problem with it. Plate pre-read measurements were made in a Costar UV plate. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 2. Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. Thermo Scientific Microplate Reader Verification Plates Cat# 24072800. MICROPLATE READER: Used, in good condition, working microplate reader. The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. Catalog Number: M5. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. For top-reading microplate readers (SpectraMax M2, M5, FlexStation II, and FlexStation 3) is it fine to leave the plate lid on? Will the excitation light get reflected off the lid in an unpredictable way?The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. . : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. i3x. 8. Self Service Available. Be sure to insert the purple (SpectraMax M5 or M5e reader) or black (FlexStation 3 reader) plate adapter in the plate carriage prior to Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. Fluorescein 0. 6% for the on-board optics (monochromators). Italic and boldface type are used for emphasis. Table of Contents. book Page 21 Wednesday, July 7, 2010 2:09 PM. Figure 1. We would like to show you a description here but the site won’t allow us. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 orThe Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader and computer with preload software (StakMax Microplate Handling System/racks not included). Click SoftMax Pro Software User Guide. Validation test plates for Abs, FI, Lum optical performance. Choose monochromator-based optics for flexibility, filter-based optics for sensitivity, or both…BioTek’s patented Hybrid Technology™ offers high-performance and applications versatility in a modular platform to expand as your laboratory’s. Read Modes Normal and Sweep Absorbance Measurement Range 0. This. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. The SpectraMax® ABS Plus Microplate Reader can run . The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). We show how the DuoLuc reporter assay and SpectraMax iD5 reader are used to detect nuclear factor-κB (NF-κB) activation in a mammalian cell model. 1. High-performance. Shake. #655801. 8. Molecular Devices SpectraMax M5 Microplate Reader is available from The Lab World Group. Or, with the reader specifications noted from the Instrument Description section of your reader's manual, as well as those for your PC, you can calculate needs more closely. 2. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. A dialog box will appear, requesting you to confirm that the read is a pre-read. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. The resulting OD 426 values are visually represented above as a color map generated in the plate section of SoftMax. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. Wokingham, Berkshire RG41 5TS United Kingdom. PURPOSE. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. (128) 100%. 314 low lamp level warning. See SpectraMax® Microplate Reader User Manual Download Page. The M5e Reader is easily automated. The standard curve was plotted using a log-log curve fit in the SoftMax® Pro Software (r2 = 1. 40266 Email: drugdiscoverytech@lifetech. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. Rating: 5. 40266 Email: drugdiscoverytech@lifetech. +44 118 944 8000. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. 2018. The SpectraMax M5 e reader has the same performance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF. Information on how to reserve the plate readerThe GloMax® Explorer is a high-performance multimode plate reader that allows you to get up and running quickly, generating the data you need from your experiments. Recertification is recommended every two years. spr SpectraMax iD3 SpectraTest ABS2 v1. We therefore gathered salient. Manufacturer: Molecular Devices. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. The dual monochromator optics allow. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. 4. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. Detection of pro-inflammatory factorsThe SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. The Molecular Devices Spectramax M5 Multi-Mode Plate Reader brings you the power and precision of a dedicated single mode plate reader, but with the space saving flexibility of 5 detection modes in one unit. 1. edu The content of this site is published by the site owner(s) and is not a statement of advice, opinion, or information pertaining to The Ohio State University. 04 to 3. Gemini XPS. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. ZERO BIAS - scores, article reviews, protocol conditions and morePage 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. REF - For the SpectraMax Plus 384, press to do a Cuvette Reference correction method read of buffer, water, or air taken in the cuvette that is used as I to calculate Absorbance or % Transmittance with the PathCheck Pathlength Measurement Technology. Download PDF. Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. Third this recommendation. Molecular Devices SpectraMax Plus 384. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell concentration left to right starting at Col. ". An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD5 reader. For SpectraMax L or LMax II, LMax II. The M5 can read volumes down to 2uL with the. spr * SpectraMax i3(x) SpectraTest ABS2 v1. Supported Plates and Cuvettes. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. There is currently no charge for using this plate reader. D 3 1. The FlexStation 3 reader’s optics are designed to easily adapt to changes in assay requirements while maintaining the optimal performance of a single-mode reader. TR-FRET assay. Trends in Immunology. The plastic from a microplate will affect the light polarization, precluding bottom reads and reading a covered plate. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 6 explains the lower values on the LmaxII384 plate reader. Note: Check the Applies to section at the bottom of the page to make this is the proper driver for your plate reader. SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. Please refer to the Obsolete Microplate Reader Models for more information. Supersedes 1. TR-FRET assay principle.